Preparation and validation of PCR-generated positive controls for diagnostic dot blotting.

نویسندگان

  • M H Liang
  • D R Johnson
  • L J Wong
چکیده

References 1. Loeffelholz MJ, Lewinski CA, Silver SR, Purohit AP, Herman SA, Buonagurio DA, Dragon EA. Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction. J Clin Microbiol 1992;30:2847–51. 2. Bass CA, Jungkind DL, Silverman NS, Bondi JM. Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens. J Clin Microbiol 1993;31:2648–53. 3. Bianchi A, Scieux C, Brunat N, Vexiau D, Kermanach M, Pezin P, et al. An evaluation of the polymerase chain reaction Amplicor Chlamydia trachomatis in male urine and female urogenital specimens. Sex Transm Dis 1994;21: 196–200. 4. Mahony JB, Luinstra KE, Sellors JW, Pickard L, Chong S, Jang D, Chernesky MA. Role of confirmatory PCRs in determining performance of Chlamydia Amplicor PCR with endocervical specimens from women with a low prevalence of infection. J Clin Microbiol 1994;32:2490–3. 5. Skulnick M, Chua R, Simor AE, Low DE, Khosid HE, Fraser S, et al. Use of the polymerase chain reaction for the detection of Chlamydia trachomatis from endocervical and urine specimens in an asymptomatic low-prevalence population of women. Diagn Microbiol Infect Dis 1994;20:195–201. 6. Catry MA, Borrego MJ, Cardoso J, Azevedo J, Santo I. Comparison of the Amplicor Chlamydia trachomatis test and cell culture for the detection of urogenital chlamydial infections. Genitourin Med 1995;71:247–50. 7. Toye B, Peeling RW, Jessamine P, Claman P, Gemmill I. Diagnosis of Chlamydia trachomatis infections in asymptomatic men and women by PCR assay. J Clin Microbiol 1996;34:1396–400. 8. Bauwens JE, Clark AM, Stamm WE. Diagnosis of Chlamydia trachomatis endocervical infections by a commercial polymerase chain reaction assay. J Clin Microbiol 1993;31:3023–7. 9. Pasternack R, Vuorinen P, Kuukankorpi A, Pitkäjärvi T, Miettinen A. Detection of Chlamydia trachomatis infections in women by Amplicor PCR: comparison of diagnostic performance with urine and cervical specimens. J Clin Microbiol 1996;34:995–8. 10. Quinn TC, Welsh L, Lentz A, Crotchfelt K, Zenilman J, Newhall J, Gaydos C. Diagnosis by AMPLICOR PCR of Chlamydia trachomatis infection in urine samples from women and men attending sexually transmitted disease clinics. J Clin Microbiol 1996;34:1401–6. 11. Peterson EM, Darrow V, Blanding J, Aarnaes S, de la Maza LM. Reproducibility problems with the AMPLICOR PCR Chlamydia trachomatis test. J Clin Microbiol 1997;35:957–9. 12. Amplicor Chlamydia trachomatis test package insert. Branchburg, NJ: Roche Diagnostic Systems, 1993 and 1995. 13. de Barbeyrac B, Pellet I, Dutilh B, Bébéar C, Dumon B, Géniaux M. Bébéar C. Evaluation of the Amplicor Chlamydia trachomatis test versus culture in genital samples in various prevalence populations. Genitourin Med 1994; 70:162–6. 14. Kellogg JA, Seiple JW, Klinedinst JL, Stroll ES, Cavanaugh SH. Improved PCR detection of Chlamydia trachomatis by using an altered method of specimen transport and high-quality endocervical specimens. J Clin Microbiol 1995; 33:2765–7. 15. Verkooyen RP, Luijendijk A, Huisman WM, Goessens WHF, Kluytmans JAJW, van Rijsoort-Vos JH, Verbrugh HA. Detection of PCR inhibitors in cervical specimens by using the AMPLICOR Chlamydia trachomatis assay. J Clin Microbiol 1996;34:3072–4. 16. Miyashita N, Iijima Y, Matsumoto A. Evaluation of the sensitivity and specificity of polymerase chain reaction test kit, AMPLICOR Chlamydia trachomatis. Microbiol Immunol 1994;38:81–5. 17. de Barbeyrac B, Rodriguez P, Dutilh B, LeRoux P, Bébéar C. Detection of Chlamydia trachomatis by ligase chain reaction compared with polymerase chain reaction and cell culture in urogenital specimens. Genitourin Med 1995;71:382–6. 18. DiDomenico N, Link H, Knobel R, Caratsch T, Weschler W, Loewy ZG, Rosenstraus M. Cobas AMPLICOR: fully automated RNA and DNA amplification and detection system for routine diagnostic PCR. Clin Chem 1996;42:1915–23. 19. Cobas Amplicor method manual for Chlamydia trachomatis, Ver. 1. Branchburg, NJ: Roche Diagnostic Systems, 1997:20. Preparation and Validation of PCR-generated Positive Controls for Diagnostic Dot Blotting, Min-Hui Liang, Dennis R. Johnson, and Lee-Jun C. Wong ( Molecular Diagnostic Laboratory, Institute for Molecular and Human Genetics, Georgetown University, Washington, DC 20007; 2 Dept. of Pathology and Pediatrics, Childrens Hospital, Los Angeles, CA 90027; * present address: Yale University, School of Medicine, New Haven, CT 065208066; † author for correspondence: Molecular Diagnostic Laboratory, Institute for Molecular and Human Genetics, 3800 Reservoir Rd., NW, Rm M4000, Georgetown University Medical Center, Washington, DC 20007; fax 202-7841770, e-mail [email protected])

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عنوان ژورنال:
  • Clinical chemistry

دوره 44 7  شماره 

صفحات  -

تاریخ انتشار 1998